The biopolymer-based from cassava starch (CS) and cellulose fiber (CF) from sugar cane leaves was prepared by help of carboxylated styrene-butadiene rubber (CSBR) as an adhesion. The CF was isolated by using acid hydrolysis under heating (50 wt%, 140 °C for 2 h). The size, morphology, chemical structure, interaction bonding, dynamic mechanical behavior, thermal analysis, biodegradation, physical and mechanical performance of CSBR/CS/CF composites were investigated. The highest tensile value was found at 1.6 wt% CF loading (27.96 MPa) while preserving the large extent of their biodegradability (<80% after buried in soil for 30 days). Moreover, the increasing CF content led to decrease in the water contact angle, moisture content owing to the interfacial adhesion between carboxyl groups on the CSBR and hydroxyl groups on the CF surfaces. This study demonstrated a significant concentration of the CF on film properties. Moreover, these inexpensive biodegradable composites were preferable for packing, fruit container and paper coating applications.